Due to the fact that the bulk of the lipid bilayer of biological membranes is phospholipids, the purpose of the researches, the results of which are presented in this paper, was to study the phospholipid composition and its cryogenic changes during preservation of bulls, rams and boars sperm. Ram gametes are characterized by a high content of phosphatidylcholine and phosphatidylethanolamine. The minor components of boar gametes are phosphatidylserine; ram - sphingomyelin and bull - phosphatidylserine and cardiolipin. By thin-layer chromatography it was found that phospholipids of gametes are represented by the following fractions: 1. Phosphatidylserine; 2. Phosphatidylcholine; 3. Sphingomyelin; 4. Phosphatidylethanolamine; 5. Cardiolipin. Of these, the largest amount is contained in the fractions of phosphatidylcholine and phosphatidylethanolamine, the content of the remaining fractions is slightly lower, namely phosphatidylserine, sphingomyelin and cardiolipin. With the passage of the technological stages from dilution to thawing of biological material, the phospholipids content gradually decreases.