Introducere: Vasele sangvine decelularizate (VSD) ar fi un material atractiv pentru grefa la pacientii cu boala arteriala periferica
avansata care necesita interventie chirurgicala de bypass, dar nu au un vas autolog adecvat de calibru mic (˂6,0mm). La
moment, au fost deja dezvoltate numeroase tehnici de decelularizare(DC), desi o procedura de operare standard nu este inca
disponibila. Actualmente nu este clar daca acelasi protocol de DC poate fi aplicat cu succes pentru vase de diferit calibru.
Scopul: Studierea eficacitatii unei metode de DC pentru dezvoltarea matricelor acelulare si evaluarea duratei expunerii la detergenti
ionici (SDS, SDC - 6h/12h/18h/24h), necesare pentru eliminarea completã a celulelor din aorta vs artera carotida.
Material si metode: Aorta porcina æi carotidele au fost tratate cu detergenti si DNaza sub rotatie. Eficacitatea a fost evaluata prin
coloratia 4’,6-Diamidino-2-fenilindol (DAPI) si Hematoxilina-Eozina (H&E).
Rezultate: Coloratia H&E nu a evidentiat celule persistente in nici un grup, inclusiv in probele tratate doar 6h cu SDS si SDC. Cu toate
acestea, coloratia DAPI a acelorasi specimene a descoperit cantitati substantiale de ADN rezidual. Prelucrarea timp de 12h cu
detergenti a permis DC completa a arterei carotide, in timp ce DC aortei a necesitat o expunere de 24h la detergenti.
Concluzii: Rezultatele obtinute sugereaza ca coloratia H&E nu poate fi utilizata ca unica dovada a DC æi ar trebui completata, cel putin,
cu o coloratie AND, precum DAPI. In plus, vasele mari necesita prelucrare mai lunga decat cele mici æi, prin urmare, nu poate fi
recomandat niciun protocol comun de decelularizare.

Introduction: Decellularized blood vessels(DBVs) would be an attractive graft material for patients with advanced peripheral arterial
disease who require bypass surgery, but do not have suitable autologous small-caliber vessel (˂6,0mm diameter). Numerous
decellularization (DC) procedures have been developed already; nevertheless, a standard operating procedure is still not available.
So far, it is unclear whether the same DC protocol can be applied successfully to BVs independent of their diameter.
Purpose of the study: Investigation of the effectiveness of a DC method for the development of acellular vessel matrices and the
duration of exposure time to ionic detergents (6h/12h/18h/24h), namely SDS and SDC, required to fulfill complete cell elimination
from aorta vs carotid artery.
Material and Methods: Porcine aorta and carotid arteries were treated with detergents and DNase under rotation. Efficacy of DC was
evaluated by 4’,6-Diamidino-2-phenylindole(DAPI) and hematoxylin and eosin(H&E) stainings.
Results: H&E staining revealed no persisting cells in all groups, including the samples treated just for 6h with SDS and SDC. The
DAPI stain of the same specimens, however, uncovered substantial amounts of residual DNA. A 12h treatment with detergents led
to a complete DC of carotid artery specimens, whereas aorta samples required a 24h exposure to the detergents. Conclusions: The
findings suggest that the H&E staining cannot be used as a sole proof of DC and should be supplemented, at least, with a DNA stain
like DAPI. In addition, large diameter BVs require an extended processing time than small diameter BVs and therefore no common
decellularization protocol can be recommended.