Ischemic stroke (IS) is a rare but underestimated disease in children. The incidence of IS is 2 – 13:100000 children or 1:4000 in neonatal period. Aim: evaluation of the expressivity of immune parameters in children with IS to improve understanding of pathogenesis, early diagnosis and predictive factors of the disease. In 2017 − 2019 in the Republic of Moldova a prospective study was carried out on a sample of 53 children with IS (study sample, SS), investigated by ELISA in the acute phase of the process determining the serum levels of endogline CD105 (ENG), S100B protein, vascular endothelial growth factor (VEGF), ciliary neurotrophic factor (CNTF), antiphospholipid antibodies (APA), and interleukin 6 (IL-6). These markers were also appreciated in 53 “practically healthy” children (control sample, CS). Six months after IS, serum levels of VEGF and S100B were re-assessed. In our study, we assessed ENG serum levels in children in the acute IS phase revealing that in SS the mean value is significantly lower than in CS (F=84,812, p<0,001), the maximum values was 4,02 ng/ml, and the minimum was 1,88 ng/ml. Unlike ENG, the level of the S100B protein in the acute phase of the disease is higher in SS than in the CS. So, in SS the mean value of S100B was 0,524±0.0850 ng/ml with a maximum value of 4,390 ng/ml, while in CS the mean level was 0,120±0,0038 ng/ml and maximum level attains the value of 0,149 ng/ml, which show a statistically significant difference between samples (F=9,330, p<0,01. Compared to CS where the average VEGF level was of 185,50±12,039 pg/ml with a maximum value of 287,44 pg/ml, which we appreciate as lower than the minimum level of this factor recorded in SS. The observed difference between two samples was statistically significant (F=60,701, p<0,001). In children from SS an average CNTF level of 7,84±0,322 pg/ml was assessed, having a very wide variation in peak values from 5,46 pg/ml to 20,26 pg/ml, and in CS the mean value was 5,29±0,067 pg/ml, with variation within smaller limits from 4,94 pg/ml to 5,90 pg/ml, (F=32,550, p<0,001). In SS were recorded a mean APA values of 1,37±0,046 U/ml compared to the mean level of 0,92±0,021 U/ml recorded in CS (F=60,701, p<0,001). In SS was determined the mean serum level of IL-6 of 22,02±2,143 pg/ml, ranging from 4,58 pg/ml to 65,71 pg/ml, showing the presence of inflammation in IS. However, in CS the mean value was 10-fold lower (2,38±0,302 pg/ml), with variations from 0,01 pg/ml to 5,38 pg/ml, which determine significant statistical difference (F=43,810, p<0,001). In presented study it was found that in the acute period of IS in children are observed changes in values of some biomarkers, and usually, the more serious the patient's condition, the higher the levels of some biomarkers and lower levels the others. Thus, the results of the study suggest that by targeting some markers of IS it is possible to analyze the inflammation, neuroprotection, vasculo- and neuroregeneration from the onset of cerebral ischemia, in order to decrease the risks of developing early and remote neurological complications and to predict their development.