Polyethylene, the most common plastic, is highly recalcitrant and inert synthetic material, and very difficult to degrade in the environment. The natural degradation of low density polyethylene (LDPE, PE-LD) depends on polymer properties, such as water insolubility, the hydrophobicity, degree of crystallinity, and molecular weight, and a number of various environmental factor including temperature, air humidity, moisture content, pH, solar energy. In the process of biodegradation, microorganisms influence the destruction of synthetic polymers by secreting extracellular enzymes and free radicals able to cleave polymer into oligomers, dimers and monomers. One of the most reliable approaches to achieve polyethylene degradation is selection of microorganisms using the polymer as sole carbon source. The microorganisms isolated previously in the laboratory of Soil Microbiology and stored in the National Collection of Non-Pathogenic Microorganisms (CNMN) were selected according to their capacity to grow and accumulate biomass in the presence of LDPE. There were tested 19 fungal strains: 7 representatives of the genus Penicillium spp., 8 – Aspergillus spp., 2 – Fusarium spp., Rhizopus arrhizus, and Cladosporum cladosporioides. For submerged cultivation, a mineral salt medium (MSM) was selected, 0.1% glucose was added to the medium as a growth inducer. The LDPE film was added to the culture medium in the form of 2 × 2 cm squares, 3 pieces per 100 ml medium. Cultivation was performed under conditions of continuous stirring, on a stirrer 180-200 rpm, at a temperature of 28C. After 40 days of submerged cultivation, the amount of microbial biomass (BM) and LDPE destruction was determined by the gravimetric method. The micromycetes that most actively produce BM in the presence of polyethylene were determined: Aspergillus flavus, Penicillium vermiculatum, P. verrucosum CNM-FP-02, Fusarium sp. 110 and F. gibbosum CNMN-FF-01 – 0.89-1.04 g/l. The grade of LDPE destruction in general was insignificant and had not exceeded 0.25-0.26%. The optical microscopy showed the immobilization of microorganisms on the surface of the plastic films and traces of damage on the surface of polyethylene from the contact with microbial cells. The ability of microorganisms to grow in the presence of LDPE was studied on solid medium Czapek’s Agar with glucose. Cultivation was performed in a thermostat at t = 28C for 30 days. Surface cultivation of Penicillium spp. with polyethylene enhanced the biodegradation of LDPE up to 4.98% on the average. Penicillium corylophilum CNMN-FP-04 was noted as the most active destructor, the dry weight of LDPE decreased by 6.06%. The average grade of LDPE destruction caused by members of genus Aspergillus was 0.69%. The direct contact between microbial cells and surface of polyethylene films increased the grade of biodegradation up to 1.03% for Aspergillus alliaceus CNM-FA-01 and 1.41% for Aspergillus wentii correspondingly. Visual observations and optical microscopy revealed the active growth of microorganisms on the surface and beneath the polyethylene films.