Effect of Phenol on the Activity and Temperature Characteristics of Peptidases in Chironomid Larvae at Different рН Values
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KUZ'MINA, Victoria V., CHORNAYA, E., SHEPTITSKY, Vladimir. Effect of Phenol on the Activity and Temperature Characteristics of Peptidases in Chironomid Larvae at Different рН Values. In: Inland Water Biology, 2021, nr. 2(14), pp. 231-238. ISSN 1995-0829. DOI: https://doi.org/10.1134/S1995082921020097
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Inland Water Biology
Numărul 2(14) / 2021 / ISSN 1995-0829 /ISSNe 1995-0837

Effect of Phenol on the Activity and Temperature Characteristics of Peptidases in Chironomid Larvae at Different рН Values

DOI: https://doi.org/10.1134/S1995082921020097

Pag. 231-238

Kuz'mina Victoria V.1, Chornaya E.2, Sheptitsky Vladimir2
 
1 Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences,
2 T.G. Shevchenko State University of Pridnestrovie, Tiraspol
 
Disponibil în IBN: 8 iunie 2021


Rezumat

The effect of phenol (Ph, 0.25 mmol/L) on the activity and temperature dependence of casein- and hemoglobin-lytic peptidases functioning in the whole organism of chironomid larvae Chironomus sp. within the temperature range of 0−70°С at different рН values (3.0, 5.0, and 7.4) is studied. The temperature optimum of the studied peptidases of chironomid larvae corresponds to 40°С. Under the influence of phenol, the activity of casein-lytic peptidases increases more significantly at 20°С and рН 5.0 (by a factor of 3.3) and that of hemoglobin-lytic peptidases increases more significantly at рН 3.0 (by a factor of 3.5). The effect successively decreases from рН 3.0 up to рН 7.4. under both temperatures. In the presence of phenol, the shape of the curves of the temperature dependence and the Q10 values transform most significantly in the zone of high temperatures. The values of activation energy (Eact) of the process of hydrolysis of casein and hemoglobin-lytic peptidases in the zone of low and high temperatures are different. In the presence of phenol, Eact values of the process of hydrolysis of casein casein-lytic peptidases at рН 7.4 increase; at рН 5.0 they decreases in the zone of high temperatures. The values of Eact of the process of hydrolysis of hemoglobin-lytic peptidases decrease in most cases. The mechanisms of the influence of phenol on peptidase activity and digestive processes in benthivorous fish feeding on chironomid larvae are discussed.

Cuvinte-cheie
chironomidе larvae peptidases phenol temperature dependence рН