In vitro evaluation of Lavandula Augustifolia and Hippophae rhamnoides extracts on promotion of bone marrow mesenchymal stem cells proliferation
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JIAN, Mariana, COBZAC, Vitalie, GÎRBU, Vladilena, MORĂRESCU (CHETRARU), Olga, ORGAN, Adina, COJOCARU-TOMA, Maria, COTELEA, Tamara, CIRIMPEI, Octavian, KULCIŢKI, Veaceslav, NACU, Viorel. In vitro evaluation of Lavandula Augustifolia and Hippophae rhamnoides extracts on promotion of bone marrow mesenchymal stem cells proliferation. In: New frontiers in natural product chemistry.: A destiny on the altar of research. Dedicated to academician Pavel Vlad, Ed. 6, 21 mai 2021, Chișinău. Chișinău, Republica Moldova: Tipografia "Artpoligraf", 2021, Ediția 6, p. 31. ISBN 978-9975-3336-7-2. DOI: https://doi.org/10.19261/nfnpc.2021.ab24
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New frontiers in natural product chemistry.
Ediția 6, 2021
Conferința " New frontiers in natural product chemistry."
6, Chișinău, Moldova, 21 mai 2021

In vitro evaluation of Lavandula Augustifolia and Hippophae rhamnoides extracts on promotion of bone marrow mesenchymal stem cells proliferation

DOI: https://doi.org/10.19261/nfnpc.2021.ab24
CZU: 57.085:[582.949.26+582.866]:54.057

Pag. 31-31

Jian Mariana1, Cobzac Vitalie1, Gîrbu Vladilena2, Morărescu (Chetraru) Olga2, Organ Adina1, Cojocaru-Toma Maria1, Cotelea Tamara1, Cirimpei Octavian1, Kulciţki Veaceslav2, Nacu Viorel1
 
1 ”Nicolae Testemițanu” State University of Medicine and Pharmacy,
2 Institute of Chemistry
 
Proiect:
20.80009.8007.03 Noi substanțe cu potențial preventiv și terapeutic în baza compușilor naturali de origine vegetală și a matodelor moderne de sinteză organică
 
Disponibil în IBN: 28 mai 2021


Rezumat

Chemical substances of natural origin represent an efficient cell proliferation tool and a lot of studies have been reported in this direction [1]. In particular, natural terpenoids showed effective in numerous studies on wound healing and other tissue engineering [2]. Lavender (Lavandula Augustifolia) and sea-buckthorn (Hippophae rhamnoides) are two plants broadly used in folk medicine for wound healing and other therapeutic and preventive purposes, predominantly in the form of essential or fatty oils [3,4]. Few studies report on the cell proliferative effect of other extracts derived from these abundant natural sources. The aim of this investigation is to test in vitro the action of extracts of Lavandula Augustifolia wastes and Hippophae rhamnoides seeds in concentration of 500 μg/mL, 100 μg/mL, 20 μg/ml and 4 μg/mL on mesenchymal stem cells from rabbit bone marrow. The cells isolation was performed according to the method proposed by Cobzac and the authors [1] with the positive opinion of the Etics Committee of 14.12.2016, no.31. Cell viability was determined by the MTT test after Mosmann [2]. According to the results of the MTT test, in all extracts compared to the control, the highest cell viability is attested at concentration of 4 μg/ml at interval of 24, 48 and at 72 hours the viability exceeds 100% which denotes a potential positive effect on cell viability over time. It should also be noted that the least cytotoxic action has the extract of Hippophae rhamnoides both at concentrations of 4 μg/mL and 20 μg/mL compared to other extracts, which would allow their more detailed research on in vivo model.