Changes In The Antimicrobial Properties Of Streptomyces Canosus Cnmnac-02 And Its Variants During Long-Term Storage By Subculturing
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BURTSEVA, Svetlana, BYRSA, Maxim, SHIBAEVA, Irina V., SHIBAEV, Alexandr, CEBOTARU, Nicola, CODREANU, Svetlana. Changes In The Antimicrobial Properties Of Streptomyces Canosus Cnmnac-02 And Its Variants During Long-Term Storage By Subculturing. In: Analele Universitatii din Oradea, Fascicula Biologie, 2021, vol. 28, pp. 20-26. ISSN 1224-5119.
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Analele Universitatii din Oradea, Fascicula Biologie
Volumul 28 / 2021 / ISSN 1224-5119

Changes In The Antimicrobial Properties Of Streptomyces Canosus Cnmnac-02 And Its Variants During Long-Term Storage By Subculturing


Pag. 20-26

Burtseva Svetlana1, Byrsa Maxim1, Shibaeva Irina V.2, Shibaev Alexandr2, Cebotaru Nicola1, Codreanu Svetlana1
 
1 Institute of Microbiology and Biotechnology ,
2 Institute of the Electronic Engineering and Nanotechnologies "D. Ghitu"
 
Disponibil în IBN: 6 aprilie 2021


Rezumat

The paper deals with the research results on changes in antimicrobial activity of strain Streptomyces canosus CNMNAc- 02 and its 3 variants (obtained using γ irradiation, combined (γ and UV) mutagenesis, low-frequency and low intensity magnetic field) after long-term storage (20 years) by subculturing. Antimicrobial activity was determined by the disk diffusion method, as test cultures were chosen opportunistic pathogenic bacteria and fungi which cause crop diseases. Analysis of the results obtained allow us to recommend oat agar medium for long-term storage of the original S. canosus CNMN-Ac-02 strain with antibacterial activity against phytopathogenic bacteria, and Czapek medium with glucose to preserve antifungal activity. For S. canosus CNMN-Ac-03, the antifungal activity is preserved on Gause medium, and for S. canosus CNMN-Ac-04 - on Czapek medium. For variant treated with magnetic field, higher antibacterial activity was registered against Clavibacter michiganensis 13a on Gause medium, by cca. 11.0 % more than original strain. Also it was noted, that strains synthesize several antibiotics, because of ability to inhibit develop both of phytopathogenic fungi and bacteria 

Cuvinte-cheie
Antimicrobial activity, Long-term storage, organic media., Streptomyces canosus, Subculturing, Synthetic media