Articolul precedent |
Articolul urmator |
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Ultima descărcare din IBN: 2024-04-02 21:28 |
SM ISO690:2012 PAVLOVSCHI, Elena, STOIAN, Alina, MALCOVA, Tatiana, IORDĂCHESCU, Rodica, VEREGA, Grigore. Decelularizarea combinată a allogrefei osoase vascularizate. etapă de studiu experimental in vivo. In: Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, 21-23 octombrie 2020, Chişinău. Chişinău: USMF, 2020, p. 519. |
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Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu” 2020 | ||||||
Congresul "Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”" Chişinău, Moldova, 21-23 octombrie 2020 | ||||||
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Background. Tissue transplantation is a successful approach to rebuild the osteoarticular defects. Critical bone defects remain a dilemma for reconstructive surgery. Decellularization of organs, including bone, gives an acellular biological graft, which keeps their extracellular three-dimensional structure. Objective of the study. To extract the cellular component from the vascularized bone allograft by the combined method, according to the algorithm, without injuring the extracellular structure and matrix. Material and Methods. The bone segment was taken from the domestic rabbit. The femur was taken with the internal iliac artery, located between the upper part of the great trochanter and the distal 1/3 of the femoral shaft, respecting the vascular continuity. The graft was processed, gradually, with a series of solutions, during mechanical agitation. Results. The decellularization process was applied according to the established protocol. Used decellularizing agents were physical, chemical, and biological. They assured the efficient removal of cellular content from the tissue, without damaging the three-dimensional structure of the extracellular matrix. Another point of the present process was the hierarchy in the elimination of components. The greatest part - the cells, were removed first, and then the protein and lipid residues. In the last step, the smallest compartments DNA and RNA, were eliminated. The grafts were examined radiologically and morphologically. Conclusion. The combined process of decellularizing of vascularized bone tissue can generate bone grafts devoid of immunological agents. The decellularized vessel needs additional studies to evaluate its resistance, an imperative factor in subsequent grafting. |
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Cuvinte-cheie vascularized bone allograft, combined decellularization, allogrefă osoasă vascularizată, decelularizare combinată |
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