The article examines the evolution of the development of the entrepreneurial ecosystem of the Republic of Belarus in the context of the entrepreneurship support infrastructure. The article provides a literary review of the definition of «entrepreneurship support infrastructure» and determines the relationship with the development of the entrepreneurial ecosystem. The author’s definition of «entrepreneurship support infrastructure» is proposed. The evolution of development of entrepreneurship support centers and business incubators in Belarus is analyzed, various legislative acts of the Government of the Republic of Belarus and the President of the Republic of Belarus in this area are considered. The presented study of the Republic of Belarus is based on author’s long-term analysis of the entrepreneurship support infrastructure in the country, as well as the experience of assessing the effectiveness of individual organizations. It is noted that the entrepreneurship support infrastructure has several stages of development, which reflect global trends in its development.