Fish are among the most important groups of wild species, from the ecological but also from the economic perspective, representing a valuable food resource for both humans and wildlife. In the same time, fish are recognized bioindicators for the assessment of aquatic pollution with different substances. Thus, it is possible to monitor the dynamics of heavy metals concentrations in aquatic organisms for the assessment of water pollution. In order to avoid a polluting disaster, such as the one in Minamata, Japan, manifested by the chronic mercury and cadmium intoxication of human population, following the consumption of contaminated fish, continuous monitoring of heavy metals concentration in fish is required. Therefore, the aim of the present study is to determine the level of different heavy metals (Pb, Cd, Ni, Cu, Fe, Zn) and metalloid (As) in 3 components (water, sediment and fish) of a single pond production system. Samples of water, sediments and fish (Silurus glanis, Cyprinus carpio and Carrassius gibelio) were collected in april 2016 from a aquaculture pond (Mălina Pond) situated in the South-East part of Romania. The analysis of heavy metals concentrations was realised by atomic absorption spectrometry (Analytik Jena, ContrAA 700), flame and graphite furnace tehnique. The results point out the following accumulation descending trend in: water – Fe (299 μg.L-1)>Zn>Ni>As>Pb>Cu>Cd (0.014 μg.L-1), sediments – Ni (36.153 μg.g-1)>Cu>Fe>As>Pb>Cd>Zn (0.128 μg.g-1) and fish muscle tissue – Fe (14.83±1.48 μg.g-1 in catfish, 19.64±4.79 μg.g-1 in carp, 18.02±0.56 μg.g-1 in prussian carp)>Zn>Cu>Pb>Cd>As (LOD = below the detection limit). The estimated daily intake (EDI) was calculated for each analysed metal as it follows: EDICd = 0.003 for catfish, 0.004 for carp, 0.001 for prussian carp, EDIPb = 0.006 for catfish, 0.007 for carp, 0.004 for prussian carp, EDICu = 0.749 for catfish, 0.663 for carp, 0.093 for prussian carp, EDIZn = 1.828 for catfish, 3.5 for carp, 5.33 for prussian carp. The EDI values were below the maximum tolerable values recommended by the WHO (World Health Organization) and FAO (Food and Agriculture Organization of the United Nations). The main conclusion of this research is that the fish produced in Mălina Pond is safe for human consumption, from the heavy metals content point of view. Also, the present paper can help identify specific heavy metals accumulation trends in the water environment in future research.