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635.64:631.524.65:575.113 (1) |
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SM ISO690:2012 ZAMORZAEVA-ORLEANSCAIA, Irina, BAHŞIEV, Aighiuni, MIHNEA, Nadejda. Molecular diagnosis of Candidatus phytoplasma solani infection in some tomato genotypes at the different ontogeny stages. In: Oltenia - studii si comunicari stiintele naturii, 2017, nr. 1(33), pp. 48-52. ISSN 1454-6914. |
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Oltenia - studii si comunicari stiintele naturii | |||||
Numărul 1(33) / 2017 / ISSN 1454-6914 | |||||
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CZU: 635.64:631.524.65:575.113 | |||||
Pag. 48-52 | |||||
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This paper presents the results of the molecular diagnosis of Candidatus phytoplasma solani infection in plants of three tomato varieties, Elvira, Desteptarea, Cerasus, created at the Institute of Genetics, Physiology and Plant Protection of the Moldavian Academy of Science. The dynamics of the appearance of stolbur infection was displayed in the process of plant development in the field conditions. Using molecular detection (nested PCR analysis) allowed to establish that phytoplasma infection was not present in the tomato plants in the field at the early stages of plant development. The first signs of the infection were registered at the beginning of the fruit ripening. About a half of plants were infected with Ca. p. solani when fruits ripened on I-III racemes. The most abundant phytoplasma infection was registered at the late ontogeny stages of the tomato: mature fruits on III, IV plant racemes. Clear differences in resistance were found between the three studied varieties to phytoplasma infection. The variety Elvira demonstrated significant sensitivity to this kind of infection in comparison with the varieties Desteptarea and Cerasus. The most resistant to Ca. p. solani variety was Cerasus - an abundant infection in its plants was registered only at late ontogeny stages, more specifically, at the end of the vegetative period. The obtained results confirm the usefulness of molecular diagnostic techniques for breeders in the creation of tomato varieties resistant to phytoplasma infection |
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Cuvinte-cheie molecular diagnosis, tomato, Candidatus phytoplasma solani, ontogeny stages, resistance, diagnosticul molecular, tomate, Candidatus phytoplasma solani, etape de ontogeneză, rezistenţă |
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