Характеристика фитопатогенной нагрузки семян озимой пшеницы устойчивого к болезням сорта куяльник при помощи молекулярно-генетических методов
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КУЗНЕЦОВА, Ирина, БЕЛОУСОВА, Галина. Характеристика фитопатогенной нагрузки семян озимой пшеницы устойчивого к болезням сорта куяльник при помощи молекулярно-генетических методов. In: Protecţia plantelor - realizări şi perspective, Ed. 57, 27-28 octombrie 2020, Кишинев. Кишинев: "Print-Caro" SRL, 2020, nr.57, pp. 346-350. ISBN 978-9975-3472-0-4. DOI: https://doi.org/10.53040/9789975347204.83
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Protecţia plantelor - realizări şi perspective
nr.57, 2020
Simpozionul "Protecţia plantelor - realizări şi perspective"
57, Кишинев, Moldova, 27-28 octombrie 2020

Характеристика фитопатогенной нагрузки семян озимой пшеницы устойчивого к болезням сорта куяльник при помощи молекулярно-генетических методов

DOI: https://doi.org/10.53040/9789975347204.83
CZU: [575.852+581.2]:633.11:632.95

Pag. 346-350

Кузнецова Ирина, Белоусова Галина
 
Институт генетики, физиологии и защиты растений
 
Disponibil în IBN: 28 octombrie 2020


Rezumat

The aim of this work was to test the seeds of the Kuyalnik variety (harvest of 2019) for the presence of pathogens of the genera Fusarium, Myrothecium, Alternaria, Penicillium, and Aspergillus by molecular genetic methods (PCR, nested-PCR). The use of species-specific primers for these pathogens allowed to detect 6 species of Fusarium (F. verticillioides, F. avenaceum, F. oxysporum, F. equiseti, F. sporotrichioides, F. incarnatum) in the seeds of the disease-resistant variety of winter wheat. It was found that the seeds of this variety of winter wheat also contained a large amount of Alternaria alternata, and a trace amount of Penicillium spp. Phytopathogens of the Myrothecium spp. genus and of the Aspergillus parasiticus were not identified in the DNA isolated from the seeds of the winter wheat variety Kuyalnik. The presence of such a diversity of fungal pathogens in the seeds of the resistant winter wheat variety Kuyalnik requires treatment of seeds with fungicides.

Cuvinte-cheie
winter wheat, seeds testing, molecular genetic methods, PCR, nested-PCR, fungal pathogens

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<dc:creator>Cuzneţova, I.I.</dc:creator>
<dc:creator>Belousova, G.G.</dc:creator>
<dc:date>2020</dc:date>
<dc:description xml:lang='en'><p>The aim of this work was to test the seeds of the Kuyalnik variety (harvest of 2019) for the presence of pathogens of the genera Fusarium, Myrothecium, Alternaria, Penicillium, and Aspergillus by molecular genetic methods (PCR, nested-PCR). The use of species-specific primers for these pathogens allowed to detect 6 species of Fusarium (F. verticillioides, F. avenaceum, F. oxysporum, F. equiseti, F. sporotrichioides, F. incarnatum) in the seeds of the disease-resistant variety of winter wheat. It was found that the seeds of this variety of winter wheat also contained a large amount of Alternaria alternata, and a trace amount of Penicillium spp. Phytopathogens of the Myrothecium spp. genus and of the Aspergillus parasiticus were not identified in the DNA isolated from the seeds of the winter wheat variety Kuyalnik. The presence of such a diversity of fungal pathogens in the seeds of the resistant winter wheat variety Kuyalnik requires treatment of seeds with fungicides.</p></dc:description>
<dc:source>Protecţia plantelor - realizări şi perspective (nr.57) 346-350</dc:source>
<dc:subject>winter wheat</dc:subject>
<dc:subject>seeds testing</dc:subject>
<dc:subject>molecular genetic methods</dc:subject>
<dc:subject>PCR</dc:subject>
<dc:subject>nested-PCR</dc:subject>
<dc:subject>fungal pathogens</dc:subject>
<dc:title>Характеристика фитопатогенной нагрузки семян озимой пшеницы устойчивого к болезням сорта куяльник при помощи молекулярно-генетических методов</dc:title>
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