Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
423 0 |
SM ISO690:2012 ERHAN, Raul Victor, BODNARCHUK, Viktor, RADULESCU, Aurel, CAZACU (ANGHEL), Lilia. Small Angle Neutron Scattering Reveals Dimeric Glucose Oxidase from Aspergillus niger at pH 5.9. In: Journal of Surface Investigation, 2020, nr. 14, pp. s5-s10. ISSN 1027-4510. DOI: https://doi.org/10.1134/S1027451020070125 |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Journal of Surface Investigation | |
Numărul 14 / 2020 / ISSN 1027-4510 /ISSNe 1819-7094 | |
|
|
DOI:https://doi.org/10.1134/S1027451020070125 | |
Pag. s5-s10 | |
Rezumat | |
Glucose oxidase (GOx) is a 160 kDa flavoenzyme dimer belonging to the family of glucose-methanol-choline oxidoreductases. Despite the abundant availability of information regarding the structure and mechanisms of interactions of GOx, there is still considerable interest in studying the properties of this protein to extend its bio-applications. The present study aims at investigating the conformational stability of GOx from Aspergillus niger in the optimal environment that presumably preserves its functional properties using small angle neutron scattering method. This method allowed computing the low-resolution three-dimensional models of the protein. Obtained results indicate protein dimerization in buffer solution pH 5.9, with a maximum particle dimension, Dmax, of 110 Å and Rg of 34.50 ± 0.025 Å. |
|
Cuvinte-cheie Aspergillus, glucose, Glucose Oxidase, Glucose sensors |
|
|