From the rhizosphere of tomatoes were isolated bacterial cultures demonstrating antifungal properties. There was carried out a series of tests after which the bacterium was identified as Bacillus subtilis, they were given worker numbers - S2 and S4. Bacteria have been reported to grow as colonies, stroke or continuous lawn on the surface of solid cultivation media, depending on the mode of spread (inoculation). The cultural morphology features of bacteria growth were studied on the conventional media: MPA, PDA, M9. Both cultures gave good growth on the represented media, which differ in specific features typical for this isolate. Bacillus subtilis S2 gave the certain well-defined colonies only on medium IPA, but on the other two media the colonies blurred, blurring the boundary of the colony. Bacillus subtilis S4 grew as sharply defined colonies in all of the three media. In this paper, we describe the differences in bacterial growth when inoculated by streak. Bacterial growth in the liquid media also had characteristic features: the presence of sediment and biofilm, pigmentation of cultivation media.