The solution of the problems of cryopreservation of sperm of farm animals is determined largely by the intermolecular
interactions of cellular components of reproductive cells. Therefore, the aim of the research was the study of the
contribution of the lipid components in the manifestation of adaptive-compensatory changes of gametes of animals in
hypothermic conditions. Experimental investigations were carried out using sperm of the boars of Large White breed,
the roosters of Rhode Island Red breed and the bulls of Black and White breed. Isolation of plasmatic membranes of
spermatozoa was conducted according to the method developed by Ivanov and Porfiry (1981) in our modification
(Наук et. al, 1993). The obtained results proved that the phospholipids in the process of cryopreservation of sperm of
farm animals are exposed to the greatest changes, while the cholesterol content is more stable. Also, the molar ratio
phospholipids:cholesterol was changed in the direction of value “1” after cooling and freezing of sperm. Decrease of
the ratio phospholipids:cholesterol is one of the mechanisms in the system of adaptive-compensatory reactions of
spermatozoa at the influence of low temperatures. The study of the adaptation mechanisms at the cellular and
molecular levels attracted the attention of an increasing number of researchers, because in this direction is possible
prospects of solving the problems of сryogenic changes during preservation of sperm.