The phenomenon of growth activation of the industrial strain of mycelial fungus S. fibuligera Y-436 under the influence of squalene and anolyte water has been investigated. Squalene had a stimulating effect on the growth of the strain on YEPD Broth in all doses. The stimulating effect of squalene was maintained for 54 hours, in a dose of 0.0005% - up to 78 hours of the strain cultivation under aerobic conditions. The growth rate of the strain in the exponential phase between 4 and 6 hours on medium with a squalene of 0.0005 % is 0.30, 0.001 % - 0.44, 0.002 % 0.41, on medium without squalene - 0.24. Squalene promotes faster adaptation of the strain to the substrate and reduces the duration of its lag phase of growth by 2-4 hours. The number of cells increases by 2.2-2.3 times with the use of squalene in doses of 0.0005-0.001 % after 24 hours and by 1.4 times after 48 hours of incubation on the nutritional mixture based on wheat flour. The strain reached the stationary phase after 24 hours of cultivation with the use of squalene, while in the control variant - after 48 hours. This reduces the duration of cultivation by 2 times. Anolyte water contributed to the rise of generative activity of the strain due to the acid reaction by 34.3 % in comparison with the initial water. The most effective method of activation of the strain is the combined use of anolyte water and squalene in a dose of 0.0005-0.001 %.