Background. Metabolic disorders, including those of the carbohydrate metabolism, are important elements of the pathogenesis of numerous diseases. More interest in the usage of different biologic active compounds, which could exert significant influence on metabolic processes, aroused currently. Local bioactive compounds (BC)  Schiff bases, their combination with 3d metals, manifest important biologic activity [1, 2], but their influence on carbohydrate metabolism in spleen in ethylene glycol (EG) intoxication was not studied. The aim of the study was to elucidate the peculiarities of spleen carbohydrate metabolism in ethylene glycol intoxication and after its remediation with local BC. Methods. Carbohydrate metabolism was studied in spleen in EG intoxication by measuring the activities of lactate dehydrogenase isoforms  LDH-L (catalyzes the conversion of lactate into pyruvate) and the LD-P (catalyses the conversion of pyruvate to lactate), as well as the functionality of the glucose-6-phosphate dehydrogenase (G6P DH) – the source of reducing equivalent NADPH+H+, and anabolic NADP-dependent malate dehydrogenase (MDH-NADPd) by spectrophotometric micromethod adapted to the Hybrid Multi-mode Microplate Rider Synergy H1, "BioTek" (USA). Treatment of ethylene glycol poisoning was carried out with local BC: Schiff bases, their combination with 3d metals  CMD-4, CMD-8, CMJ-23, and cyanobacterial remedy  BioR-Ge, with enhanced content of organically bound Ge. Results. Experimental EG intoxication induced the decrease of LDH-P and G6P DH activities, while the functional level of NADP-MDH increased slightly compared with reference values. CMD-4 had led to the normalization of the activity of both LDH isoforms, while BioR-Ge reduced it. CMD-8 and CMJ-23 kept low LDH-P. Medication with CMD-8 and BioR-Ge returned to normal values, while CMJ-23 induced statistically conclusive the functional level of G-6-P DH activity. CMD-4, CMD-8, CMJ-23 and BioR-Ge increased MDH-NADPd activity in the spleen of animals with EG intoxication. Conclusions. Studied local BC exhibit the capacity to normalize the activity of the majority of carbohydrate metabolism enzymes in spleen. The selective action of the tested compounds, which probably depends on their degree of engagement at different stages of metabolism, is obvious. Studied BC can be used as pathogenic remedies for the correction of metabolic disorders, which are specific for the ethylene glycol induced toxic lesions of the spleen. Research conducted in this area would allow to develop new methods of efficient and harmless treatment of various immune system disorders that would ensure a high therapeutic effect and preventive action in respect to severe complications.