Diagnosis and evaluation of Vicia Faba L. germaplasm resistance to biotic stresses
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BALASHOVA, Irina, PIVOVAROV, Victor, PRONINA, Ecaterina, GRECOV, Ivan, GORDEEV, Dmitrii, KINTIA, Pavel, MASHCENKO, Natalia. Diagnosis and evaluation of Vicia Faba L. germaplasm resistance to biotic stresses. In: Biotehnologii avansate – realizări şi perspective: Simpozionul ştiinţific naţional cu participare internaţională, 24-25 octombrie 2013, Chişinău. Chișinău, Republica Moldova: Tipografia Academiei de Ştiinţe a Moldovei, 2013, Ediția III-a, p. 77.
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Biotehnologii avansate – realizări şi perspective
Ediția III-a, 2013
Simpozionul "Biotehnologii avansate – realizări şi perspective"
Chişinău, Moldova, 24-25 octombrie 2013

Diagnosis and evaluation of Vicia Faba L. germaplasm resistance to biotic stresses


Pag. 77-77

Balashova Irina1, Pivovarov Victor1, Pronina Ecaterina1, Grecov Ivan1, Gordeev Dmitrii1, Kintia Pavel2, Mashcenko Natalia2
 
1 All-Russian Research Institute of Vegetable Breeding and Seed Production,
2 Institutul de Genetica şi Fiziologie a Plantelor
 
 
Disponibil în IBN: 21 martie 2020



Teza

The broad bean (Viciafaba L.) is one of the most ancient crops in the world. It is represented in collections by cultivated forms only, because the wild ancestor of its species hasn’t yet been discovered (Gerard Duc et al., 2010). Investigation of Viciasubgenus phylogeny based on analysis of RAPDs and RFLP of PRC-amplified chloroplast genes show, that broad bean could been introduced in Russia from 2сenters of origin: from Asia (India, Afghanistan) and from Europe (The Mediterranean region) (Potokina E.K. et al., 2008). Viciafaba L. have been planted in Russia from VI century. Broad beans are cultivated at the northern and the central areas of Russian Federationnow, and we have 5 varieties of broad bean at present (Pivovarov V.F., 2010). But the importance of broad bean is increasing now, because: 1)it’s seeds are rich in protein and starch, 2) its play the vast role in the improvement of the soil, 3) its are need for private vegetable-gardens in Russia. But Vicia faba L. are infected with biotic stressors as all Fabaceae. And that moment is weakening of its positions on the food Fabaceae market (Vishniakova M.A., 2008). So, we began the special program for the broad beans breeding on resistance in All-Russian Research Institute for Vegetable Breeding and Seed Production. The first step of the program was the diagnosis of diseases infected broad bean at the Middle part of Russia and preliminary evaluation of plants resistance to diseases. We evaluated the germaplasm broad bean of our Institute: 5 varieties, 20 samples from the collection of VIR and 20 breeding samples. Broad beans were infected with Uromycesfabae (Grev.) de Bary ex Fuckel, Ascochytafabae Speg. and broad bean mottle virus. Uromycesfabae (Grev.) de Bary ex Fuckel (syn.Uromycesvicia-fabae (Pers.) Schröter). The fungus commonly attacks broad bean, but has a wider host range on various species of Vicia, Lathyrus and Lens (Conner R.L., Bernier C.C., 1982). Plants show typical rust pustules on the upper and under sides of leaves and on stems. Aescospores may overwinter in the Mediterranean region, but can’t to survive in severe winters of our region. Mycelium, uredinio- and teliospores in leaves and stems could to remain viable for 1-2 years. The degree of disease spreading was 76% in 2009 (the humid summer) and 6,5% in 2011 (the dry summer). Ascochytafabae Speg. A. faba resembles A. pisi, but it is largely specialized to Vicia faba L. It causes large lesions (up to 10 cm in diameter) on leaves and more small by diameterspots on stems and beans. Lesions have reddish-brown margins and grayish centers with black dots of pycnidia in the center. Colorless pycnospores have cylindrical form and 1-2 partitions. Infected seeds are covered with circular dark brown spots. Pathogen can remain viable into seed for up to 3 years and for 4-5 months on infected trash (Dixon G.R., 1984). The degree of disease spreading was 100% in 2009 (the humid summer) and 1,5% in 2011 (the dry summer). Broad bean mottle virus caused vein clearing and chlorotic mosaic of leaves. The upper part of leaf was pointed. The degree of disease spreading was 23% in 2009 and 46, 3% in 2011. Unfortunately, we couldn’t to support of our visual diagnosis with help of ELISA. So, we think that our studies will in progress. Significant negative correlation between the degree of plants affection and the degree of seed germination has been established (r= -0,49; tr= 3,50> t05 = 2,01). We show that steroid glycoside ecostim (c= 0, 005%) could to stimulate the embryo roots growth of 2 broad bean varieties: Russkiechiornie and Byelorusskie.