Генетический контроль поддержания хиазм в мейозе у томата
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576.354:582.926.2 (1)
Cellular and subcellular biology. Cytology (130)
Systematic botany (855)
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ЛИСОВСКАЯ, Татьяна. Генетический контроль поддержания хиазм в мейозе у томата. In: Genetica, fiziologia şi ameliorarea plantelor: conferinţă ştiinţifică internaţională , Ed. 6, 23-24 octombrie 2017, Chişinău. Chişinău: "Print-Caro" SRL, 2017, Ediția 6, pp. 125-128. ISBN 978-9975-56-463-2.
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Genetica, fiziologia şi ameliorarea plantelor
Ediția 6, 2017
Conferința "Genetica, fiziologia şi ameliorarea plantelor, "
6, Chişinău, Moldova, 23-24 octombrie 2017

Генетический контроль поддержания хиазм в мейозе у томата

CZU: 576.354:582.926.2

Pag. 125-128

Лисовская Татьяна
 
Восточноевропейский национальный университет имени Леси Украинки
 
 
Disponibil în IBN: 18 decembrie 2019


Rezumat

A comparative analysis of the cytological manifestation in the meiosis of six meiotic mutants of tomato was carried out. Buds with anthers 2-3 mm long fixed in ethanol: glacial acetic acid in a ratio of 3:1, were stored in 70% ethanol, stained with acetocarmine. For cytology investigations we prepared pressure anthers at various stages of meiosis. Pollen fertility was determined by staining with acetocarmine. Meiotic mutations tomato dsm1, dsm2, dsm3, as1, as5 and asb not affect the vegetative growth of plants, but have low fertility of pollen and number of seeds in the fruit. They are similar cytological manifestation. The beginning of meiosis occurred evidently normal, as violations of premature decay bivalent observed since diplotene. Chiasma frequencies in pollen mother cells ranged from 8.13 in dsm3 / dsm3 to 6.73 in asb / asb. All investigated mutations revealed monogenic recessive character of inheritance. Mutations as1, as5 and asb and dsm2 are not allelic to each other. The mutation dsm1 appeared allelic to mutations dsm2 and asb. Investigated meiotic mutations failure of chiasma maintenance in the pollen mother cells with varying degrees of desynapsis. Since previously published data did not reveal a decrease in the recombination frequency in the marked areas of the genome, we assume premature removal of cohesin, which keeps sister chromatids together distally to chiasmata.  

Cuvinte-cheie
meiosis, meiotic mutants, sister chromatids cohesion, Lycopersicon esculentum Mill