Thiosemicarbazones derived from 2-formylpyridine and their metal complexes exhibit awide range of biological applications. Nevertheless, the literature contains examples of biologically important bidentate thiosemicarbazones: p-acetamidobenzaldehyde thiosemicarbazone, known as thiacetazone, is employed in the treatment of tuberculosis [1]. There are some reports on the studies of 4-phenyl 2-formylpyridine thiosemicarbazones and their metal complexes with antioxidant properties that surpass 33 times better than Trolox [2]. Using the procedures previously described for synthesizing thiosemicarbazones and their metal complexes as well as measuring their antifungal activity. The group of researchers [3] have tested coordination compounds of copper (II) with 4-allylthiosemicarbazone of 2-acetylpyridine and showing an antifungal activity 133 times better than Fluconazole. Based on published data suggesting that the acetanilide and pyridin-2-yl fragments present biological properties. Aims of this work are synthesis of new ligands that will contain 4-phenylacetamido-3thiosemicarbazones derived from 2-formylpyridine. The tridentate ligands containing derived from 2-formylpyridine thiosemicarbazones were synthesized and characterized by 1H, 13C-NMR, FTIR spectroscopy. On the basis of the ligands and copper salts obtained ten coordination compounds, characterized by FTIR spectroscopy. Coordination compounds could look potential biological activity for antimicrobial, antifungal and antioxidant properties.