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CHIORCHINA, Nina; GORCEAG, Maria; SOFRONII, Maria; CRISTIAN, Cristina; TROFIM, Mariana. Micropropagation protocol of Paulownia through in vitro culture technique. In: Conservation of plant diversity. Ediția 4-a, 28-30 septembrie 2015, Chișinău. Chișinău: Gradina Botanica (Institut), 2015, p. 60. ISBN 978-9975-3036-8-2.
|Conservation of plant diversity
Ediția 4-a, 2015
Simpozionul "Conservation of plant diversity" |
Chișinău, Moldova, 28-30 septembrie 2015
Paulownia tomentosa, Paulownia elongata, Micropropagation, tissue culture
Paulownia is a genus that belongs to the Paulowniaceae family (previously to the family Scrophulariaceae) and includes 6-17 species of plants. They are native to China and have been naturalized in other parts of the world such as USA and Europe. The genus is receiving increasing attention as an extremely fast growing, short-rotation woody crop plant, with large leaves arranged in opposite pairs on the stem. The trees are used for re-forestation, roadside planting and as ornamental trees. They grow well in a wide variety of soil types, notably poor ones, and yields a multiple-purpose wood, has potential medicinal use and because of its wide-spreading root system can be used for phytoremediation of contaminated soils. The Paulownia tree adapts easily to a wide range of climatic conditions, such as, temperature and a range of soils. It grows well in abundance of sunshine and rainfall, usually on sandy and clay soils. A high demand for planting material in domestic and international markets for afforestation and bioenergy has determined the development of efficient micropropagation protocols for rapid mass propagation of Paulownia. Due to their rapid growth and value in the timber market, many Paulownia species are cultivated in several temperate zones worldwide. Therefore, the application of biotechnological approaches for in vitro regeneration and micropropagation techniques of Paulownia have been encouraging, particularly for supply of planting material for forestry. The use of in vitro propagation techniques provides healthy, homogeneous planting stock for a forestation and woody biomass production of Paulownia . The aim of this study was to develop an effective in vitro regeneration protocol for micropropagation of two Paulownia genotypes (using Murashige and Skoogand), study the consecutive micropropagation behaviour, multiplication, risogenesis, in order to improve plantlets production quantitatively and qualitatively. The research was conducted in the Embryology and Biotechnology Laboratory, within the framework of the Botanical Garden (Institute) of the ASM. As biological material were used two Paulownia species (P. elongata and P. tomentosa). Explants were collected from tissues of mature plants. The shoot tips and nodal segments were cut and collected in a beaker containing water to avoid desiccation and then brought to the laboratory. The shoot tips (15-20 mm length) were sterilized, were washed thoroughly under running tap water for 15 minutes, then with liquid detergent with few drops of Tween-20 (polyoxyethylene sorbiton monolaurate) and finally rinsed several times in distilled water then with a solution 0,001% KMnO4 for 15 minutes. After repeated washing with distilled water, the explants were finally treated with 0.1% diacid for 10 minutes and with 5% H2O2 for 5 minutes in the laminar air flow cabinet and washed for three times with autoclaved double-distilled water. After disinfection, the axillary buds were excised and inoculated, one by one, into each test tube . Initial explants were cultivated on basal medium MS 100% and supplemented with metatopolin (0.5 mg/l). For induction of roots, well-developed single plants were put on MS 50%. The subculture period was 45 days in growth chambers with permanent temperature +25 Co white fluorescent light with 3000 Lux intensity and 16/8 h photoperiod . Based on our results, the following conclusions can be drawn: the studied Paulownia genotypes were successfully propagated in vitro and significant multiplication was achieved. Application of the propagation medium with MS and addition of meta-topolina resulted in high proliferation and induced development of uniform plants as a prerequisite for effective rooting and high quality material production. Successful in vitro rooting was achieved when plants were cultivated on MS 50%. High average efficiency of adaptation (96%) was obtained.