|Ultima descărcare din IBN:|
| SM ISO690:2012|
CHIORCHINA, Nina; SEDCENCO, Maria; JOSU, Mihail; CUTCOVSCHII-MUŞTUC, Alina. Introduction and micropropagation of cultivars of the Genus lycium. In: Conservation of plant diversity. Ediția 4-a, 28-30 septembrie 2015, Chișinău. Chișinău: Gradina Botanica (Institut), 2015, p. 59. ISBN 978-9975-3036-8-2.
|Conservation of plant diversity
Ediția 4-a, 2015
Simpozionul "Conservation of plant diversity" |
Chișinău, Moldova, 28-30 septembrie 2015
Lycium barbarum, introduction, in vitro propagation, MS medium, adaptation
In recent years, in the Republic of Moldova, the market demand for new assortments of crops has increased, contributing directly to the implementation of the Food and Health Programme of the country. The creation of industrial plantations with new species of fruit-bearing shrubs is a novelty for the Republic of Moldova and plays an important role in the implementation of the programme. Berries or “small fruits” are characterized by high content of vitamins, minerals and antioxidants. They contain a class of compounds called “the 21st century vitamins”, vitamins which include flavanols, anthocyanins, dimers and trimers thereof, called “catechins” and polymers with high molecular weight, tannins. Large-scale cultivation of fruit-bearing shrubs and especially of new, less frequently cultivated species contribute to the introduction of new species into culture and provides a source of healthy food with a wide range of fruits. Lycium barbarum, also known as Chinese wolfberry or Himalayan goji is native to China, but was also introduced into Europe [Tămaş M., Ilioara Oniga 2009; Fernandez F. F. 2008]. Researches on the regeneration of some new cultivars of Lycium barbarum by vitro cultures are conducted, within the framework of the institutional project, by the team of Embryology and Biotechnology Laboratory. This shrub became popular lately due to the taste of its fruits, their therapeutic properties and rich content of biologically active substances; this fact explains the interest of people in high quality planting material of this crop. The use of modern biotechnology in agriculture ensures the production of planting material of high biological value that meets important criteria: genetic uniformity, quality of planting material and price, and it is also a method of obtaining pathogen-free planting material. This project aims at the development and optimization of in vitro propagation technologies for new species of fruit-bearing and ornamental shrubs, one of which is Lycium barbarum L. New results of the research on micropropagation and obtaining healthy planting material are expected by applying and consolidating scientific results obtained, in this field, by the scientific community from Europe and from Moldova, in particular. The problem addressed in this project has two aspects of novelty and originality: first – the new species which are studied in order to be cultivated later and second – the studies on micropropagation of these species. The cultivar with high productivity, of L. barbarum L. was propagated in vitro through apical meristem. The sterilization method, the terms of sampling and the size of explants were tested. The MS 100% culture media supplemented with different concentrations of cytokinins (BAP, KIN), which cause the development of adventitious shoots, were established and tested. After 4 weeks since inoculation, the inoculated material was inventoried, from six types of media, the agar medium supplemented with 0.5 BAP stood out. The inocula from this medium provoked callus formation, from which, multiple adventitious shoots developed, about 14.1 per explant. The obtained micro-plantlets were transplanted for rhizogenesis to the agar medium ½ MS without hormones; during 2 weeks, the root system developed and, during 10 days, the plants matured and grew 6-10 cm tall with 2-3 internodes for in vitro microfragmentation and the basal part, with roots – for ex vitro. At the moment, the plants are tested for adaptation and acclimatization to ex vitro conditions.