The effect of cyanobacterial zinc sulfated polysaccharides from Arthrospira (Spirulina) platensis CNMN-CB-02, as a supplement added in complex liquid medium M-I, on accumulation of biomass, synthesis of total lipids and fractional lipid composition of strain S. canosus CNMN-Ac-02 was studied in laboratory conditions. Maximal quantity of biomass compared to control was obtained using 30.0 %/V Psh*ZnS (8.83 g/l) concentration, with an increase of 63.91 %. In the experiment total lipids were lower than in the control. However, no significant difference was observed at 5.0 %/V Psh*ZnS concentration (95.82 %) in comparison to control. At all concentrations of Psh*ZnS applied, fractions of phospholipids and sterols were higher than those observed in the untreated control. Significant increase of triglycerides (20.63 %) was obtained adding in the growth medium 10.0 %/V Psh*ZnS. All tested concentrations of Psh*ZnS had no effect on mono- and diglycerides in comparison to control, whereas waxes were significantly lower. Quantity of sterol esters was higher in comparison to control only at concentrations of 1.0-20.0 %/V Psh*ZnS. Total lipids were increased by the increase of biomass, by 15.9 % in comparison to control, after the addition of Psh*ZnS at 30.0 %/V concentration.