The study of morphology and anatomy of Withania somnifera (L.) Dunal plants, obtained by biotechnology in vitro, and grown in the conditions of the Republic of Moldova were investigated. It was elucidated the morphological specific characteristics for root and herba (stems, leaves, flowers and fruits). The results of anatomical structure of the root, stem, leaf, flower and fruit of W. somnifera showed that the vascular bundles of root and stem are open, collateral and provide the secondary structure; there are the oxalate calcium as sand in the root cortex and rosette in the leaves and calyx; abundant multicellular, dendroid trichomes on stem, leaves and calyx; rarely secretory hairs on the lower epidermis of leaf; lacuna in the stem cortex and sclerenchymatic fibre periciclic ring in the stem. Their morphological and anatomical structures have a diagnostic role in taxonomy identification and some adaptive peculiarities may be suitable