The adhesion and antagonist capacity of some Enterococcus strains isolated from human and animal intestinal contents was investigated. The obtained results demonstrated the increased adhesive capacity of the studied Enterococcus strains, especially those specific to the human digestive tract. At the same time, the high activity of enterococci monocultures in the control of Escherichia and Salmonella bacteria has been established, which indicates their antagonistic property. Based on the adhesive capacity and high antagonistic activity of enterococci, new microbial associations containing enterococci were investigated. The obtained results revealed a beneficial action of the new microbial preparations, which prevented the appearance and development of diarrheal dysfunctions, which argues the opportunity to include enterococci in the composition of associations or probiotic microbial preparations.