The paper presents the results of research on the acclimatization of vitroplants of Vaccinium vitis-idaea L. and Vaccinium macrocarpon Aiton (Fam. Ericaceae) obtained by in vitro culture. For the adaptation of the studied species, several types of substrate were tested. The highest yield of acclimatized plants (70-80%) was the acid peat substrate (pH 3.5-5.0). Another decisive factor is the maintenance of high atmospheric humidity, light intensity and the optimum temperature for the development of neoplants (21 ° C-25 ° C).