Currently, nanomaterials based on inorganic nanoparticles are widely used in biology, medicine, biotechnology, food industry, and agriculture due to their unique properties to exhibit enzyme-like activity. The relevance of this report is determined by the fact that diagnostic devices for the specific detection of biological and chemical targets at low concentrations in a reliable, convenient and inexpensive manner Of great interest is the design of sensitive sensors based on nanozymes, i.e., enzymatic nanomaterials, such as ZnO, Fe3O4, and ZnFe2O4. These materials have high sensory properties and can catalyze the reaction of the peroxidase substrate to change the color of the 3,3',3,5'-tetramethylbenzidine (TMB) dye [1]. In our study, nanoparticles ZnO of size 30-40 nm were by the sol – gel method and with the solvatothermal method obtained ZnFe2O4 with a dimension of 5–6 nm with poly(N-vinylpyrrolidone) (PVP) as a stabilizer. The obtained nanoparticles were characterized by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). ZnO gives us very good photocatalytic properties, which affect the catalytic reaction occurring in the presence of ZnFe2O4 nanocomposite, these photocatalytic properties will contribute to accelerate the reaction after creating the heterostructure underlying this work. These photocatalytic properties contribute to the reaction acceleration after the creation of the heterostructure underlying this work. Based on these nanoparticles, a heterostructure was prepared by casting and used for glucose detection. The study was conducted by colorimetric method at a wavelength of λ = 652 nm to visualize the redox reaction occurring in a model system containing solutions of glucose oxidase (GOx), dye TMB and glucose [2]. The study showed the possibility of glucose detection in the concentration range of 1 - 11 mM using the ZnO/ZnFe2O4 heterostructure.