For identifying new sources of adaptability to abiotic stress factors, a collection of tomato  regenerants  has  been  studied.  To  characterize  the  resistance  of  genotypes  to  elevated  temperature and lack of moisture, a set of gametophytic selection methods was used. The reaction of  genotypes to stress was evaluated by the variability of such signs of male gametophyte,  as  the   viability  of  pollen  and  the  length  of  pollen  tubes  in  optimal  and stressful conditions.  Statistical processing of the obtained results allowed us to establish the  differences  between   the  genotypes  for  thermal  stability  and  resistance  to  water deficiency. In addition,  differences between the regenerants and the ability to maintain a high quality gametophyte under  conditions of prolonged temperature action were found. The  field  productivity  analysis  of   plants  allowed  us  to  isolate  regenerants,  which productivity exceeded the control variety  Elvira; thus, these genotypes combine high heat resistance of the gametophyte and good productivity  indicators. Selected genotypes can be recommended for cultivation in conditions of high temperature  and lack of moisture, and  can  be  used  in  the  selection  of  parental  pairs  for   hybridization.  The  use  of  the multifactor dispersion method allowed us to analyze the structure  of the spectrum of the variability of the gametophyte traits to reveal the main factors determining  the variability, as well as their contribution to the overall phenotypic variability of the traits.  To identify the degree of conjugacy of the signs, correlations between the analyzed parameters were  determined, which in some cases turned out to be highly reliable positive, which indicates the  prospect of using these indicators in assessing and selecting resistant genotypes, as well  as  the   general  genetic  systems  controlling  these  indicators.  Thus,  based  on  a combination of  embryo culture methods, gametophyte selection, statistical analysis, and classical  breeding   approaches,  fundamentally  new  genotypes  were  obtained  that  are resistant to temperature  factors and lack of moisture while maintaining and increasing their productivity.