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SM ISO690:2012 CHESOV, Elena, CIOBANU, Nelly, KRUDU, V.. Genetic mutations associated with reduced linezolid sussceptibility of mycobacterium tuberculosis strains. In: Cercetarea în biomedicină și sănătate: calitate, excelență și performanță, Ed. 1, 20-22 octombrie 2021, Chişinău. Chișinău, Republica Moldova: 2021, p. 89. ISBN 978-9975-82-223-7 (PDF).. |
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Cercetarea în biomedicină și sănătate: calitate, excelență și performanță 2021 | ||||||
Conferința "Cercetarea în biomedicină și sănătate: calitate, excelență și performanță" 1, Chişinău, Moldova, 20-22 octombrie 2021 | ||||||
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Rezumat | ||||||
Background. Linezolid (LNZ) is one of the main drugs used for multidrug-resistant tuberculosis (MDR-TB) treatment. Current molecular based tools do not allow to test susceptibility to LNZ Genetic targets associated with resistance to LNZ are not fully characterized. Objective of the study. To identify mutations associated with LNZ resistance by applying whole genome sequencing (WGS) technique and to describe correlations between pheno-genotypic resistance in MDR M. tuberculosis (MTB). Material and Methods. Multidrug resistant MTB strains, isolated from sputum of the patients treated with LNZ containing regimen during 2017-2018 in the Republic of Moldova, were analyzed. The WGS results of the MTB strains were compared with the results of liquid culture-based susceptibility tests on 3 concentrations of LNZ (0.5 mg/L; 1.0 mg/L; 2.0 mg/L). Results. The study included 60 MDR strains of MTB. The rate of phenotypic resistance to LNZ at different concentrations of the drug was 33.3% for 0.5 mg/L; 26.7% for 1.0 mg/L; 21.7% for 2.0 mg/L. Genotypic resistance was confirmed in 16.7% MTB strains. In 7 cases mutations were detected in the rplC gene (460T> C). In other 3 cases the identified mutation implied rrl gene (2746G>A, 2814G>T, 2814G>T; 2810A>C). At LNZ concentration of 1.0 mg/L, pheno-genotypic concordance was found in 71.4% of the cases. Conclusion. LNZ resistance in the studied MTB strains was associated in most cases with mutations in the rrl and rplC genes. Further molecular based diagnostic tests should include these molecular targets. |
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Cuvinte-cheie tuberculosis, linezolid, resistance, WGS, tuberculoza, linezolid, rezistenţă, WGS |
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