Many studies are devoted to the problem of knowledge and abilities. At the same time, mechanism of it,s actualization is still unknown. This essay, using methodology of active approach, is aimed to explore psycho-pedagogical conditions of knowledge actualization. For its solving there was conducted an empirical study – series of experimental training and questionnaire. The results obtained evidence the existence of system of psychopedagogical conditions of knowledge actualization, which includes three subsystems: 1. Features of organization of digestion process which provide for the following actualization of knowledge and abilities; 2.Subsystem of condition characterizing storage method of knowledge and abilities; 3. Features of the organization of “business” activity promoting actualization of knowledge and abilities.