The purpose of the research was the study of copper bioaccumulation in spirulina biomass and its distribution in various fractions extracted from biomass. Cyanobacterium Spirulina platensis is widely explored and used in recent decades as a source of important bioactive substances. An important direction in biotechnology research is obtaining of spirulina biomass fortified with important micronutrients. In last decades action of coordination compounds of Zn(II), Co(II), Fe(III), Mn(II), Se(IV), Cr(III) on the growth and biochemical composition of cyanobacteria and microalgae has been extensively studied. Recent researches have demonstrated pronounced biological activity of coordination compounds of copper(II). Research subject served cyanobacterium Spirulina platensis CNM-CB-02. As regulators of copper content in biomass were used coordination compounds of Cu (II) acetato-N-[2-(2-hidroxietilamino)-ethyl]-salicilidenimino(1-)copper ([Cu(LH)( CH3COO)]) and bromo-{3-[(2-hydroxy-5-nitro-benzylidene)-amino]-propane1,2-diolo}(1-)copper ([Cu(L-H)Br]). As a result of the research two procedures of cultivation of spirulina in the presence of new coordination compounds of Cu(II) were developed. Cultivation of spirulina in the presence of the compounds [Cu(L-H)(CH3COO)] and [Cu(L-H)Br] at a concentration of 2,0 and 6,0 mg/L leads to reduction of productivity (1,02 and 0.92 g/L), but at the same time copper content accumulated in biomass reaches values of 10,63 and 11,14 mg%, respectively. Using fractionation of spirulina biomass, it was determined that the largest growth of copper content is in carbohydrate fraction (28% and 31%). About 30% and 27% of the total amount of copper accumulated is bound to amino acids and oligopeptides. Processes of obtaining biomass of Spirulina platensis enriched with copper bound to organic compounds could be applied to develop new technologies for obtaining copper components preparations with antimicrobial, anti-inflammatory, and anticancer activities.