Contamination of soil with heavy metals has become a serious problem in some industrial and agricultural sites. One of the most commune soil pollutant is copper. Usually high concentrations of Cu have been reported in vineyard soils due to the use of copper-based fungicide and also on wood treatment sites where in order to protect against insects and fungal a solution containing Cu, Cr, and As is used. The most important problem that occurs in copper polluted soils is the bioavailability of the element to living organisms. In general, the contaminated land is found in a large areal, and the old methods of excavation and burial of the contaminated soil are not practical, so are needed alternative methods of stabilization less invasive, with small costs, and predictable results. This objective can be achieved by using natural amendments in order to fix the contaminant in soils. It can be considered that stabilization had success if the amount of the pollutant remains constant over the time. Because the concentrations of Cu are associated with soil texture and several other parameters, in particular pH and organic matter, many remediation treatments are based on organic mixture by converting the element into less exchangeable and thus less bioavailable forms. In order to assess the efficiency of the applied amendment it was used the sequential chemical extraction (SCE). Usually SCE is fractioned, applied on a soil sample, starting with the weakest reagent and ending with the strongest one. According to Tessier, SCE has five fractions: exchangeable, carbonate bound, Fe and Mn oxide bound, organic matter bound and residual. In general metals of anthropogenic origin are found in the first four fractions and in the last fraction are found those from the parental rock. The soil samples were collected from a field experiment where for almost 70 years, in order to prevent infestation with insects and fungi, wood was treated using copper sulphate, or arsenate copper chromate. Due to the lack of legislation and guidelines regarding waste management, the soil became polluted, in particular, with Cu, Cr and As. In this case the experiment had two stages. The first stage was to immobilize the pollutants with the help of organic amendments and the second one consists in highlighting the efficiency of the applied treatments by using sequential chemical extraction. The applied organic amendments where: organic matter (OM), dolomite (DL), organic matter and dolomite (OMDL), organic matter and zero-valent Fe (OMZ). In order to confirm the treatment efficiency, and to find the fraction in which Cu becomes instable, the soil samples were analyzed by applying a particular protocol of sequential chemical extraction. After each extraction, the obtained solution was analyzed using Atomic Adsorption Spectrometry (AAS). The metal content avaible for plants is given by the mobility index. The metal concentration from the first two fractions are considered to be bioavailable and the elements found into the last tow, tend to be more stable in soils. In this study the mobility index for Cu for untreated soil was 19.27 (%) and in general for unpolluted soils, Cu has a mobility index smaller than 10 (%). The results of the SCE technique underline the fact that a high percentage of the total concentration of Cu was presented in exchangeable and organic fraction indicating an anthropogenic source of the soil pollution. Also, the application of OM treatment was the most efficient in the attempt of stabilize cooper into the soil, preventing the future leaching into the underground water and also the plant uptake.