Present study is dedicated to assessment and analysis of flood runoff and its spatial distribution in the limits of the Republic of Moldova. SCS-CN model and GIS were used as main methods and techniques for runoff assessment. Main components which were considered for flood runoff estimation are soils (soil texture and moisture condition), land cover and rainfall (for 1% probability). The results showed the region that is the most affected by flood runoff is northern part of the republic with flood runoff >100 mm, the lowest flood runoff values being estimated for the southern part of the country with <50 mm. Soil moisture conditions have a important impact on flood runoff amount, increasing its averages with 30 to 60% in case of wet condition and decreasing it with 35-65% in case of dry soil. It was also observed that flood runoff distribution is highly influenced by land cover, the average values being twice as high in urban area than under natural vegetation.