The EU the Maritime Spatial Planning Directive (2014/89/UE) aims to reduce conflicts and increase synergies among human activities, including the reduction of pressures on marine ecosystems. Involving the political choices, having in mind the best overall welfare for society, are also a priorities. Taking these into account, the spatial and temporal relations among human uses occurred in the Romanian Black Sea case study, were done, by analyzing their distribution, investigating possible interactions among them, adding the main pressures identification, exerted on the marine ecosystem, focused on marine fisheries and aquaculture. There were evaluated possible scenarios taking into account the results produced by using new methods, DISPLACE, GRID, Cumulative impact, as well as the stakeholder consultation. The resulted maps of spatial interactions between activities and the matrices of possible conflicts are presented. GIS data layers, based on derived information from various sources were created. A pairwise geographic-areaofoverlap matrix was realised and each activity was characterized by a set of factors. The computation of the conflict score was made based on a grid with corresponding cell size value equal to 1km. The distribution of spatial interactions among human uses was computed as the sum of scores for each 1x1 cell of a grid covering all study area.Through this analysis potential “hotspots” between conflicting spatial uses have been identified after including representative groups of datasets in the analysis. The marine uses evaluated, demonstrate the spatial and temporal heterogeneity and the varying use intensity of activities. Key outcomes referring to potential pairwise conflicts are discussed and could be developed, taking into account the temporal ad spatial analyses of marine aquaculture and fisheries in Romania. The results obtained will support the national authorities and decisions factors for the management of marine fisheries improvement and strengthening. Also, the National Maritime Spatial Plan, in developing according to the MSP Directive terms of implementation will include the database and sectoral plans elaborated under this study.