The purpose of this study was to determine the content of heavy metals (Pb, Cu, Zi and Ni) in acacia flowers - the main melliferous forest species in Republic of Moldova. More than 90 samples were collected from forest ecosystems, protection strips and trees on the edges of the car routes and the level of heavy metals (HM) contamination in acacia flowers was assess. With Spectroscan Maks-G was determined the level of the HM by the Roethgen-fluorescence spectrometry method. In the representative samples the level of HM fell below the maximum admissible limits (MAL) established by the World Health Organization (WHO) for medicinal plants, with the exception of two cases for Cu. This fact makes efficient to use of acacia flowers in beekeeping and pharmaceutical. In depending on the geographical area, on average, the HM values studied didn’t show significant differences, with some increasing tendencies for the central area of RM. Based on the result of the spatial distribution of the level of HM contamination of acacia flowers, we have the recommend for economic purposes for beekeepers and the entities subordinated to the “Moldsilva” Agency keep away to use of the acacia in near the industrial areas of Balti municipality, Rezina city and Chisinau municipality.