This comprehensive review examines the assessment of fungal contamination in animal feed in Sudan and explores its far-reaching implications for environment protection and natural resources management. Fungal contamination poses a critical challenge in maintaining the quality and safety of animal feed, thereby impacting both the livestock industry and the environment. Sudan's diverse agricultural practices and significant livestock sector make it vulnerable to fungal contamination in animal feed. Factors such as inadequate storage facilities, suboptimal quality control measures, and limited awareness among feed producers and farmers contribute to the prevalence of fungal contamination. This creates an environment conducive to fungal growth and mycotoxin production, posing health risks to both animals and humans. Improper disposal of contaminated feed further leads to environmental pollution, impacting soil quality, water resources, and ecosystem health. It is crucial to implement effective measures to address these challenges and mitigate the risks associated with fungal contamination. Addressing the issue of fungal contamination necessitates comprehensive strategies throughout the feed production and supply chain. Key interventions include enhancing storage facilities, implementing rigorous monitoring and testing protocols for fungal contamination, promoting the adoption of good agricultural practices, and raising awareness among stakeholders about the critical importance of feed quality for environment protection and sustainable natural resources management. The ramifications of fungal contamination in animal feed are significant for Sudan's livestock sector, as they directly impact animal health, the integrity of the environment, and the sustainable management of natural resources. This review underscores the urgency of collaborative efforts and comprehensive measures to combat fungal contamination, considering Sudan's specific circumstances and the implications for environment protection and natural resources management. By understanding and addressing these challenges, Sudan can pave the way for safeguarding animal welfare, preserving the environment, and ensuring the sustainable use of its valuable natural resources.