Articolul precedent |
Articolul urmator |
213 9 |
Ultima descărcare din IBN: 2024-05-17 05:10 |
Căutarea după subiecte similare conform CZU |
579.873.1 (3) |
Microorganisme. Bacterii (188) |
SM ISO690:2012 LUNGU, Andrei, VOLOSCHYUK, Leonid. Aspects of Saccharopolyspora spinosa cultivation and spinosad separation. In: Natural sciences in the dialogue of generations, 14-15 septembrie 2023, Chişinău. Chişinău: Centrul Editorial-Poligrafic al USM, 2023, p. 97. ISBN 978-9975-3430-9-1. |
EXPORT metadate: Google Scholar Crossref CERIF DataCite Dublin Core |
Natural sciences in the dialogue of generations 2023 | |||||||
Conferința "Natural sciences in the dialogue of generations" Chişinău, Moldova, 14-15 septembrie 2023 | |||||||
|
|||||||
CZU: 579.873.1 | |||||||
Pag. 97-97 | |||||||
|
|||||||
Descarcă PDF | |||||||
Rezumat | |||||||
For a successful agriculture, it is necessary to have at hand efficient methods of plant protection that allow obtaining high productivity without loss of quality. The greatest perspective at present a method based on the microorganism or their metabolites, which are friendly to the environment. The race with the most promise are tele actinomycetes that produce or wide range of metabolites that have not yet been widely exploited by our society. Currently, our research is focused on cultivating Saccharapolyspora spinosa to isolate from the culture liquid the metabolite called spinosad. Nutrient medium compositions containing: malt extract, soy flour, yeast extract were tested for cultivation, which previously showed very good growth of S. spinosa biomass. Large mycelial conglomerates were noted on microscopic examination. For the cultivation of S. spinosa, the method of deep cultivation on a liquid medium was used, the cultivation was carried out for 96 h, at a temperature of 30 oC. The liquid mother culture was used for inoculation, being used immediately after obtaining (the storage period of the mother culture was not studied.) For the spinosad separation, fresh culture liquid was used that was not kept for more than 24 h after the completion of cultivation. The separation was carried out through a series of repetitive centrifugations, initially to get rid of the components of the nutrient medium that were not used by the actinomycetes and later with the use of ethyl alcohol as a solvent, which is available, cheap and sufficiently safe for humans. Upon obtaining a yellow liquid, an attempt was made to crystallize spinosad by placing the liquid at temperatures of 35-40 oC under vacuum. Following the cultivation trials, it was proven that ethyl alcohol is not suitable for carrying out the separation of spinosad. In our opinion, there is a need to carry out further research with other organic solvents that will allow a better dissolution of spinosad. It is also necessary to carry out additional studies of the presence of spinosad in the culture medium to be firmly convinced that its synthesis by S. spinosa takes place. |
|||||||
Cuvinte-cheie Saccharopolyspora spinosa, separation, spinosad, cultivation |
|||||||
|