Diarrheal diseases are one of the main causes of death worldwide. The genera Salmonella and Shigella continue to be the major cause of acute diarrhea in many countries. The aim of this study was to identify pathogens from the Enterobacteriaceae family, in MTA Buiucani Public Health Medical Institution, Chisinau. The research was carried out in the microbiological laboratory during 2011-2022. 12496 clinical samples (faecal masses) were investigated. Isolation of the pure culture was carried out on the differential and selective culture media. The identification of microorganisms was carried out by biochemical tests and by the serological method of agglutination. From the total number of clinical samples investigated, 129 cases (1.03%) were found, in which the etiological agent was represented by pathogenic microorganisms from 2 genera of the Enterobacteriaceae family: Salmonella (S. Enteritidis, S. Typhymurium) and Shigella (S. sonnei, S. flexneri). Representatives of these two genera were isolated annually, the highest share had the genus Salmonella with 90.7%, and the genus Shigella – 9.3%. Analyzing the structure of the pathogenic strains identified, we found that in most cases the serovars S. Enteritidis (53.49%) and S. Typhimurium (37.21%) were isolated. The most frequently isolated Shigella serogroups were S. flexneri and S. sonnei – 4.65% each. The analysis of pathogenic strains distribution depending on the frequency of isolation, showed that the dominant serovars of the Salmonella enterica species were S. Enteritidis (83.33%) and S. Typhimurium (75%), and the species of gen. Shigella varied between 16.67% (S. flexneri) and 33.33% (S. sonnei). The highest circulation of pathogens Shigella spp. and Salmonella spp. was revealed in 2014 (2.01%), 2015 (1.78%) and 2011 (1.67%). In 2013 and 2017, the share of positive strains was practically similar, constituting about 1%, and in 2012, 2016, 2018 - 2022 - it varied from 0.12% to 0.89%. The major causes of acute diarrheal disease during the study period were pathogenic microorganisms from the Enterobacteriaceae family, representatives of the genera Salmonella and Shigella. Permanent surveillance and implementation of infection prevention strategies are necessary to mitigate acute bacterial diarrhea.