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Botanică sistematică (869) |
SM ISO690:2012 DOBROJAN, Sergiu, DOBROJAN, Galina, CIOLACU, Tatiana, CIOBANU, Eugeniu, MELNIC, Cristin. Cultivation of cyanobacteria Nostoc punctiforme (küt.). List. on different nutritive mediums. In: Natural sciences in the dialogue of generations, 14-15 septembrie 2023, Chişinău. Chişinău: Centrul Editorial-Poligrafic al USM, 2023, p. 93. ISBN 978-9975-3430-9-1. |
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Natural sciences in the dialogue of generations 2023 | ||||||
Conferința "Natural sciences in the dialogue of generations" Chişinău, Moldova, 14-15 septembrie 2023 | ||||||
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CZU: 582.232.7 | ||||||
Pag. 93-93 | ||||||
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Currently, the cultivation and exploitation of cyanobacteria is a priority direction in biotechnology and algology. Thus, the development of an efficient cianobacteria cultivation technology is necessary for the mass production of biomass that can be used in various spheres of human activity. The selection of nutrient media for the cultivation of cianobacteria represents a basic technological stage and is practically present in all cultivation technologies. Nutrients media, on the one hand, are intended to provide the algae with nutrients necessary for the growth and maintenance of the culture and, on the other hand, they directly influence the accumulation of biologically active substances in the cianobacteria biomass. This paper presents the quantitative aspects of the cultivation of the cyanobacteria Nostoc punctiforme (Küt.). Elenk. on different nutrient mediums with the aim of highlighting the optimal nutrient environment for cultivating and maintaining the culture. The experiments were carried out in laboratory conditions, the cyanobacteria was cultivated, according to the periodic method, on nutrient media Drew, BG-11 and Z-8, at a temperature of 22-25 ◦C and a light intensity of 4000 lx, for a period of 15 days. Cyanobacteria was inoculated in the amount of 0.4 g/l, the inoculum being obtained from the culture in the exponential growth state. The obtained results were processed mathematically, determining the arithmetic mean and the standard error. The pH of the culture when growing on the experimented media differs according to the cultivation medium, in all the tested variants the tendency to alkalinize the nutrient media was attested. The highest pH values were recorded when growing on Z-8 nutritive medium, and the lowest on Drew medium. The obtained results show that the highest productivity of the N. punctiforme is attested on the 12th day of cultivation on the nutritive medium Z-8 (1,60±0.08 g/l), and the lowest on the cultivation on Drew medium (0,82±0.04 g/l). When cultivating N. punctiforme on nutritive medium BG-11, the biomass reached the maximum value of 1,50±0.06 g/l on the 12th day of cultivation. It should be noted that the exponential growth phase of the culture was attested in the period of 3-12 days when growing on nutritive medium BG-11 and Z-8, and when growing on Drew medium it extended up to the 15th day. Although the cultivation cianobacteria on nutritive media BG-11 and Z-8 ensures a large amount of biomass, the culture grown on these media is more sensitive and can become infected with some algae, when reaching the stationary or regression phase, and when cultivated on the Drew nutritive medium this risk is considerably reduced. |
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Cuvinte-cheie Cyanobacteria, nutritive media, cultivation |
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