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SM ISO690:2012 KOZUB, Natalia, SOZINOVA, Oksana, SOZINOV, Igor, BLUME, Ya.. Identification of novel wheat-rye 1bl.1rs translocations using prolamin and SSR markers. In: Natural sciences in the dialogue of generations, 14-15 septembrie 2023, Chişinău. Chişinău: Centrul Editorial-Poligrafic al USM, 2023, p. 45. ISBN 978-9975-3430-9-1. |
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Natural sciences in the dialogue of generations 2023 | |||||||
Conferința "Natural sciences in the dialogue of generations" Chişinău, Moldova, 14-15 septembrie 2023 | |||||||
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CZU: 575.224.232.3:633.11 | |||||||
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Wheat-rye translocations involving arm 1RS (1BL.1RS from the rye Petkus as in the cultivar Kavkaz and 1AL.1RS from the rye Insave as in Amigo) are among the most widespread introgressions of common wheat cultivars. They carry a number of disease resistance genes (McIntosh 2013) and genes associated with root length (Gabay, 2023). Because of the loss of efficiency of the resistance genes, a promising trend is the search for novel wheat-rye translocations. The objective of our study was identification of new wheat-rye translocations. Common wheat cultivars and hybrid material were studied. Gliadins were analyzed by APAG electrophoresis. Sec-N secalins were analyzed by SDS-electrophoresis. Translocations were identified using the secalin loci Sec-1 and Sec-N, where Sec-N is located about 15 cM distally to Sec-1 (Kozub et al. 2014, 2018). Analysis of gliadins encoded by the Gli-A1 and Gli-B1 loci permitted to identify the translocation type (1AL.1RS or 1BL.1RS). Untypical translocations were analyzed by PCR with SSR markers for 1RS: proximal scm9 (Saal and Wricke, 1999) and distal Xtsm92 (Kofler et al. 2008). In the cultivar Vyshyvanka with 1BL.1RS, the pattern encoded by Sec-1 is similar to that of Gli-B1l, but it has a novel allele at Sec-N encoding two secalins in the zone of 70 kDa. The line CWX with 1RS from the rye Voronezhske SGI has specific secalin patterns encoded by both Sec-1 and Sec-N. In the cultivars Homin and MV Táltos, we revealed 1BL.1RS, but with the secalin patterns as in the cultivar Amigo. In the above cultivars and CWX, PCR with scm9 revealed a 208-bp allele, as in Kavkaz-type 1BL.1RS carriers. The distal marker Xtsm92 produced 190-bp amplicons in Homin and MV Táltos, like in Amigo-type 1AL.1RS carriers, and no amplicons in Vyschyvanka and CWX, whereas Kavkaz-type 1BL.1RS carriers showed a 185-bp band. Thus, in 1RS of the above cultivars and the line CWX, the pericentromeric portion derives from Petkus. In Homin and MV Táltos, 1RS is of recombinant origin involving the widespread Kavkaz and Amigo-type translocations. CWX and Vyshyvanka carry novel 1BL.1RS with specific alleles at the secalin and distal SSR marker loci, and they may be promising carriers of new disease resistance genes. |
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Cuvinte-cheie Triticum aestivum, secalin, 1BL.1RS, translocation, microsatellite marker |
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