Fusarium head blight is the most harmful disease of wheat. In addition to direct yield losses due to yield reduction, pathogens, fungi of the genus Fusarium, contribute to the accumulation of mycotoxins in grain. Their quantity is regulated in the European Union at the level of 1.25 mg per 1 kg. Breeding for resistance to this disease is carried out all over the world in order to obtain safe grain products and food products from it. The Czech Crop Researche Institute annually conducts research on the resistance of wheat varieties to head blight at the stage of variety testing, together with the Central Institute for Supervising and Testing in Agriculture (CISTA). Artificial infection is carried out in the flowering phase, by spraying ears with a suspension of spores of the fungus Fusarium culmorum. The susceptibility of varieties is assessed in the field after infection, taking into account the number of affected grains, the impact on the yield and the accumulation of deoxynivalenol in the grain (IFA). Wheat seeds are also analysed for Black Point damage. As a result of the research, it was found that, against an infectious background, the varieties RGT Telemark, Dagmar, Wiwa, Campesino, Mercedes showed the greatest resistance. The degree of their stability was at the level of 6-7 points according to the CISTA scale (9 points - very high resistance, 1 - very high susceptibility). The number of affected seeds in these varieties was in the range of 15.8-27.8, and the content of mycotoxin deoxynivalenol did not exceed 30 mg/kg. The average defeat of varieties was at the level of 5.7 points with 48% of grains affected by Fusarium. The maximum content of mycotoxins in susceptible varieties was 107 mg/kg. The defeat of the Black Point on a provocative background of the disease (additional irrigation of crops) was at the level of 12.7%. The varieties Wiwa, Bohemia, Balitus, Fakir, Butterfly, Partner, Pallas, LG Orlice, Crossway were the least affected. The degree of damage to the seeds of these varieties did not exceed 5%. Evaluation of yield indicators showed that Fusarium infection can reduce the weight of 1 000 seeds by up to 40% in highly susceptible varieties. Seeds affected by Black Point are generally larger and their weight compared to the control can be higher by an average of 14%.