The research results of the biotechnological potential of microbial strains with different taxonomic statuses are presented for stable microbial consortia creation in complex preparations with insect-fungicidal properties development. Six strains of microorganisms with fungicidal properties and four with insecticidal properties were selected from the Collection of the Engineering and Technological Institute "Biotekhnika" of the National Academy of Agrarian Sciences of Ukraine. Their biotechnological potential was analyzed toward test objects (phytopathogens and insects). It was found that Trichoderma fungi demonstrate the highest fungicidal activity, while Streptomyces fungi exhibit the highest insecticidal activity.