The general purpose of this article is the analysis of the stakeholders involved in organic agriculture sector in the Republic of Moldova, determining their role in this sector. Agriculture is one of the key economic sectors in the Republic of Moldova and is the main source of raw material for the agri-food industry, assure a big part of exports and income of the rural population. Orienting ourselves towards the European and other Western markets, it is necessary to mention the increasing trends in the demand for ecological products. The consumption of organic products per capita in the European Union increased from 40.8 EUR per capita in 2016 – to 63.2 in 2020, Austria has the highest consumption respectively – 177 EUR in 2016 and 254 EUR in 2020. In the world, Switzerland holds the first place in per capita consumption - 418 EUR/capita. The EU action plan mentions the need to increase organic surfaces up to 25% of the total arable land. In this context, the key actors acquire a special importance in the development of the value chain of organic agricultural production. In process of developing the article, the following research methods were applied: analysis of existing information, comparative analysis, synthesis of existing information, deduction method. Following the use of research methods, quantitative and qualitative parameters were established and the role of the key stakeholders of organic agriculture in the Republic of Moldova defined.